FastQCFastQC Report
Sat 23 Apr 2022
EGAF00006070850

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006070850
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11110654
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTGGA32700.012.7398541
CTCGTAT4600.012.71773942
ACGGGAT19800.012.6130179
AACGGGA27500.012.4357528
AAACGGG32950.012.0861737
CGATCAG4300.012.03439933
GGAAACG33900.011.6815245
GAAACGG34450.011.1684646
TCGTATG5300.011.03803643
GTCACGA5100.011.02916729
CGGGATT19850.010.88102810
TCTCGTA4850.010.66933941
CGTATGC5350.010.51430644
ACTCGCG1954.9210934E-410.3847116
GCGCGAT3858.034476E-99.93435220
CGCGATC4051.8386345E-89.44376821
CGGGAAT12250.09.1831810
GATTTCG14450.09.0312265
AAGCGGC8500.09.0012183
ACGCCCG5556.184564E-118.9182834
Produced by FastQC (version 0.11.5)