FastQCFastQC Report
Sat 23 Apr 2022
EGAF00006070855

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006070855
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8816193
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT8050.028.7768242
GATCGGA8400.027.9433081
CGATCAG8800.027.35058433
TCGTATG9100.027.1864643
TCTCGTA8650.026.78073741
GTCACGA8750.026.73170929
ATCGGAA9400.025.6754342
CGTATGC9600.025.30194944
ACACGTC10200.023.81359913
TCGGAAG10150.023.7782343
TCACGAT10400.023.142830
ACGATCA10850.022.39027832
ATCTCGT11000.022.08232740
CGTCTGA11400.021.89876216
AGTCACG11400.021.10961728
CGGAAGA12250.019.8860974
ACGTCTG13500.018.49228715
CACGTCT13600.018.19094314
CACGATC14250.017.9951531
GTATGCC14450.016.84676445
Produced by FastQC (version 0.11.5)