FastQCFastQC Report
Sat 23 Apr 2022
EGAF00006070864

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006070864
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7506404
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTCGTA6750.026.99906543
TATCTCG6850.026.27611241
CTCGTAT7150.025.17395244
ATCTCGT7800.022.78767242
TCGTATG8100.022.49922245
GATCGGA8100.021.4018341
GTCACGT8650.021.32831629
AGTCACG8700.021.2058828
ATCGGAA9300.018.634722
TCGGAAG9600.018.2868293
CGTGGCC10050.018.13370133
ACACGTC10250.017.99901813
ACGTGGC10650.017.74586332
CGTCTGA10650.017.74539216
TCACGTG11550.016.36285230
ACGTCTG11500.016.23813415
CGGAAGA11050.016.0908834
CACGTCT12450.014.99918114
CACGTGG14000.013.66015131
GCACACG14050.013.29108811
Produced by FastQC (version 0.11.5)