FastQCFastQC Report
Sat 23 Apr 2022
EGAF00006070867

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006070867
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12725478
Sequences flagged as poor quality0
Sequence length36
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTGGA32000.012.5175381
ACGGGAT21500.012.2785349
AAACGGG35750.012.1253457
AACGGGA29800.012.0296778
GAAACGG36900.011.6661586
GGAAACG36500.011.2597835
CGGGATT20700.011.23139410
AACGGGT5250.09.4281618
CGGGAAT11850.09.3666810
GCGTGAG22800.09.14512822
CCCGATT3306.1118044E-109.0918692
ACAGGCG30650.09.00450118
CCCAAAG102500.08.7964661
GAGTTCG12700.08.73977612
CGAAACG2752.4474866E-78.72689155
CCCAAGT43750.08.7099031
CGTGAGC23650.08.56276823
GAGCCAC63500.08.50430126
GTTCGAG13600.08.49227614
ATAGGCG3603.1486707E-98.3329718
Produced by FastQC (version 0.11.5)