FastQCFastQC Report
Sat 23 Apr 2022
EGAF00006070870

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006070870
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8747034
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTCGTA6750.020.65768841
CTCGTAT6850.019.69946742
CGTATGC7000.018.95420344
TCGTATG7900.016.79649543
GATCGGA7350.016.597251
ATCTCGT8350.016.16063940
CGTCTGA9200.014.91037716
CGTTGGA27050.014.7820861
ACACGTC9250.014.58658713
ATCGGAA8950.014.1273392
CGTCCGA1301.9359183E-413.8398216
AAACGGG28900.013.7727947
GAAACGG29300.013.5859356
ACGGGAT17800.013.3916019
AACGGGA24450.013.1523638
TCGGAAG9650.013.1026333
CGGGATT16500.012.81114910
ACGTCTG10900.012.58490515
CGGAAGA10100.012.29530054
GGAAACG31550.012.2375535
Produced by FastQC (version 0.11.5)