FastQCFastQC Report
Sat 23 Apr 2022
EGAF00006070874

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006070874
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9492145
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGCC682960.7195001761983197TruSeq Adapter, Index 12 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT80950.040.3190342
TCTCGTA81800.039.9279941
CGTATGC82350.039.77015744
TCGTATG82200.039.7332743
ATCTCGT82900.039.39818240
GTATGCC85000.038.6603645
GATCGGA86600.038.421791
ATCGGAA87350.038.057322
AATCTCG86150.037.91209439
CGTCTGA88750.037.53703316
TCGGAAG88850.037.413443
ACACGTC89100.037.3889913
ACGTCTG90000.036.98952515
CGGAAGA90600.036.740524
CACGTCT92150.036.20030214
GCACACG93550.035.58660511
CACACGT96600.034.57944512
GTAATCT101800.032.32681737
GTCACCT104700.031.6677529
AGTCACC104650.031.64055428
Produced by FastQC (version 0.11.5)