FastQCFastQC Report
Sat 23 Apr 2022
EGAF00006070880

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006070880
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6201649
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAACGGG19600.012.9702737
CGTTGGA18400.012.8691531
ACGGGAT12750.012.704259
AACGGGA16950.012.210828
CGGGATT11900.011.91023410
GAAACGG20950.011.8128976
AACGGGT3703.601599E-1010.944548
GGAAACG22150.010.7668075
ACGGGTT3451.735225E-79.7813529
GCTAGAC13350.09.26996729
ACGCCCG2801.4994704E-48.84035934
GACGATT2801.502099E-48.8389342
CGGGATA2607.0164824E-48.65273510
CGCCCGG6003.45608E-118.62507335
CGGGTTT4601.2814708E-67.82508210
CGGGAAT7303.45608E-117.7044910
ACCGCGC5056.4109554E-77.574444331
GATTTCG8055.456968E-127.5461015
GCGTGAG17050.07.520967522
CAAAGCG9900.07.51721861
Produced by FastQC (version 0.11.5)