FastQCFastQC Report
Sat 23 Apr 2022
EGAF00006070890

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006070890
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7740005
Sequences flagged as poor quality0
Sequence length36
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGA171610.22171820302441664TruSeq Adapter, Index 2 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA18300.028.6057781
ATCGGAA19300.027.4351022
TCGGAAG19550.027.1641563
ACACGTC19000.027.15355313
CGTCTGA19600.026.16928716
CGGAAGA20450.025.8219534
ACGTCTG20100.025.44369515
CACGTCT20550.024.88653414
GCACACG21300.024.43334611
CACACGT22300.023.53944612
AGCACAC30600.017.64469510
AGAGCAC30050.017.5706338
CAGTCAC30950.017.20242127
GAGCACA31250.016.94379
TCTGAAC31500.016.5687818
GAAGAGC32950.016.3907186
GTCACTG31900.016.36102129
GTCTGAA32650.016.21486717
CCAGTCA32650.015.75552226
AGTCACT34800.015.08379628
Produced by FastQC (version 0.11.5)