FastQCFastQC Report
Sat 23 Apr 2022
EGAF00006070897

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006070897
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6141549
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTGGA19500.014.7725741
ACGGGAT12400.014.5153969
AACGGGA17300.013.7854318
GAAACGG21050.013.681916
AAACGGG21000.013.3930537
CGGGATT12350.013.298923510
CACCGCG6100.012.90918430
GGAAACG21100.012.7965015
ACCGCGC5550.012.16174731
GCCACCG16450.011.89909628
GGCGTAA1558.5051527E-411.61231721
CGCCCGG7650.011.47075635
CCGCGCC6350.010.98387632
CGCGCCC4800.010.78096933
CGAAACG1903.873868E-410.65735710
ACAGGCG23350.010.50268218
CCCAAAG73500.010.3186621
ATCGCGC2201.3112726E-410.22742337
GCGTGAG17000.010.05831522
TCGCGCC2251.6453747E-410.00014638
Produced by FastQC (version 0.11.5)