FastQCFastQC Report
Sat 23 Apr 2022
EGAF00006070898

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006070898
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6683960
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTGGA19700.013.0463891
CGGGATT10600.011.67245110
AAACGGG19500.011.65177257
ACGGGAT11100.011.5519999
GAAACGG20100.011.1920386
AACGGGA16350.010.8696268
GGAAACG21150.010.4236775
TATGCCG2402.7765425E-510.31344345
AACGGGT3909.938958E-99.8059478
ACAGGCG25400.09.56529918
CGTTGAC1900.0045476279.4926621
ACCACGC13750.09.32798631
CCCGAAT6003.6379788E-129.018031
TCGTATG4304.8532456E-88.89609543
GCGTGAG18400.08.80317722
ACGCCCG3653.8901217E-68.63150734
ACCGCGC5504.8930815E-108.59156631
CACCACG16550.08.5656130
CGTGAGC18200.08.52908523
CGTATGC4001.4673496E-68.438271544
Produced by FastQC (version 0.11.5)