FastQCFastQC Report
Sat 23 Apr 2022
EGAF00006070899

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006070899
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7622641
Sequences flagged as poor quality0
Sequence length36
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGA173270.2273096686568343TruSeq Adapter, Index 2 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA18250.027.9892541
ACACGTC18800.026.96488413
ATCGGAA19100.026.8617952
TCGGAAG19600.026.176373
CGTCTGA19600.025.86427516
CGGAAGA19950.025.492054
ACGTCTG20050.025.20897515
CACGTCT20450.024.93591514
GCACACG21200.024.12639211
CACACGT22850.022.5155112
AGAGCAC28300.018.2333228
AGCACAC30300.017.42561710
CAGTCAC30400.017.2183527
GAAGAGC30650.017.0830616
GAGCACA30900.016.6503669
CCAGTCA31750.016.34451726
GTCACTG31500.016.33139429
TCTGAAC31750.016.202818
GTCTGAA32950.015.93134117
AGTCACT34200.015.48061828
Produced by FastQC (version 0.11.5)