FastQCFastQC Report
Sat 23 Apr 2022
EGAF00006070900

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006070900
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7073373
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAACGGG20050.014.6910867
AACGGGA17750.014.3145298
CGTTGGA21050.014.283851
GAAACGG22150.013.1965526
ACGGGAT13650.012.8486939
GGAAACG23800.012.2459675
CGCGCCC3551.4551915E-1112.03638133
CGGGATT12150.011.84406310
AGGCGTA1751.8180575E-411.56382320
ACGCCCG2851.3399949E-711.04528934
ACCGCGC4800.010.77599231
CACCGCG4800.010.77599230
CCGCGCC5000.010.34495232
CGGGAAT8500.010.31674510
ACGGGAA8150.09.9321189
CTCCGAT2109.3084073E-49.6887761
GCCACCG14700.09.63822328
CGAAGAT6400.09.1841531
GCGTGAG16100.08.93878922
CGCCCGG6301.8189894E-128.92320835
Produced by FastQC (version 0.11.5)