FastQCFastQC Report
Sat 23 Apr 2022
EGAF00006070908

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006070908
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12443910
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTGGA35250.014.6961071
AACGGGA31800.014.2920138
ACGGGAT23300.014.2914069
AAACGGG38500.013.1489477
CGGGATT22850.012.89905210
GAAACGG40050.012.8082346
GGAAACG41350.011.9158655
CGGGAAT13350.010.28066510
CGGGATA4854.1654857E-109.2781410
CTCGTAT5556.184564E-118.91853744
CCACGGA8000.08.7278111
TCTCGTA5701.09139364E-108.68383943
CGCGCCC5201.551598E-98.65333733
ACGGGAA14650.08.6004189
GTTGGAA72600.08.1592972
GATCGGA7101.8189894E-117.93076851
CACCGCG7101.8189894E-117.92206930
ACCGCGC7152.1827873E-117.866669731
CGCCCGG11300.07.7650335
TCTCGAC3552.2971124E-47.605186531
Produced by FastQC (version 0.11.5)