FastQCFastQC Report
Sat 23 Apr 2022
EGAF00006070909

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006070909
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5114921
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTACG2450.018.36705633
CGTACGT2550.017.64712334
GTACGTA3250.013.84620535
ACCGTAC3400.013.23469532
CTCGTAT3303.6379788E-1212.95636744
TCTCGTA3303.6379788E-1212.95611343
CGTTGGA13750.012.9309861
CGTAATC3701.8189894E-1212.16244638
AAACGGG13650.011.8680597
TCGTATG3753.8198777E-1111.40171345
CACCGCG3204.803951E-911.24938130
AACGGGA11250.011.1993858
CGGGATT8250.010.90849110
CCCCGAT1450.0068154310.865191
CGTATGC2503.6904657E-610.80162345
ACGGGAT8800.010.7380469
GAAACGG14950.010.6855546
GCGTGAG11050.010.58765322
TATGCCG1500.00877424910.5015793
GCCACCG10750.010.2552528
Produced by FastQC (version 0.11.5)