FastQCFastQC Report
Sat 23 Apr 2022
EGAF00006070916

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006070916
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7435015
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT9650.029.84092942
TCTCGTA10200.027.79073541
TCGTATG11000.026.17863343
CGTATGC11100.025.94279144
ATCTCGT11550.024.5421440
CATCTCG11550.024.1525839
GATCGGA12750.022.4429971
CGTCTGA13150.021.55616816
ATCGGAA13600.021.1862892
GTATGCC13850.020.62967545
TCGGAAG14350.020.0783183
ACACGTC13950.019.99810613
CGGAAGA15200.018.9555174
ACGTCTG15400.018.40734715
CACGTCT15900.018.25298914
GCACACG18200.015.57555111
CACACGT19200.014.9986812
CGTCCGA1108.570821E-414.3237616
CGTTGGA21050.013.7007781
CTTAGGC21150.013.40289533
Produced by FastQC (version 0.11.5)