FastQCFastQC Report
Sat 23 Apr 2022
EGAF00006070917

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006070917
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10182306
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT10200.027.7795644
TCTCGTA10400.027.67970543
TATCTCG10750.026.98771341
GATCGGA11550.025.6216131
TCGTATG12000.023.86788745
ATCGGAA13050.023.5284252
TCGGAAG13450.022.9966663
ATCTCGT12850.022.75228742
ACACGTC13450.022.2370913
CGTCTGA14050.020.80729516
ACGTCTG15150.019.74183815
CACGTCT15800.019.07200214
CGGAAGA16850.017.686454
GCACACG17100.017.62208411
CACACGT20300.014.84421812
ACGGGAT18150.013.7529889
CGGGATT17400.013.18255210
CGTTGGA27850.013.1403511
AACGGGA25900.012.4161518
AAACGGG29900.011.8832887
Produced by FastQC (version 0.11.5)