FastQCFastQC Report
Sat 23 Apr 2022
EGAF00006070922

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006070922
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7192738
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTGGA20450.014.6599861
AACGGGA18650.013.5098378
GAAACGG22400.013.2618136
ACGGGAT12900.013.253639
AAACGGG22550.012.8692757
GGAAACG23150.012.4503255
CGGGATT11250.012.39795610
CGGGAAT8650.010.92305510
CCCCGAT2554.5851957E-610.607581
CCCGATT2605.7822435E-610.4035881
CCGCACC5350.010.09179432
CCACGAT4702.239176E-99.1123631
CGCACCC4001.5305704E-78.99851733
CCCGATC2350.002664198.6327651
GTTGGAA41050.08.6120832
CACCGCG6951.8189894E-128.41587830
ACCGCGC6755.456968E-128.3319631
ACGGGAA9500.08.2881089
CCCCGTT3003.1059145E-48.2650721
GCCACCG17000.08.2045328
Produced by FastQC (version 0.11.5)