FastQCFastQC Report
Sat 23 Apr 2022
EGAF00006070923

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006070923
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6515548
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATGAA2757.876224E-811.4775851
CACCGCG4001.0913936E-1111.24667730
CGTTGGA20150.011.0768261
GATTTCG9300.010.4227085
AAACGGG20350.010.1689867
ACCGCGC4051.7389539E-99.99704631
CGCCCGG5201.8189894E-129.94944235
GCCACCG12900.09.93892428
ACGGGAT11950.09.7879039
CCCCGAA3002.9708663E-69.7696111
AACGGGA16500.09.6789588
ACTGCGC4458.6583896E-109.60390431
CCCCGTT3104.5121196E-69.4544631
GAAACGG22000.09.4077566
GGAAACG21950.09.3454885
CGTGAGC15400.09.05604523
CAAAGCG11250.09.0181031
GCGCCCG5007.421477E-108.99775634
AACGGGT5007.4396667E-108.9973428
CGGGATA3502.219329E-68.99734110
Produced by FastQC (version 0.11.5)