FastQCFastQC Report
Sat 23 Apr 2022
EGAF00006070930

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006070930
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7598207
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTGGA23400.012.5362451
AAACGGG25450.011.6680417
CGGGATT14150.011.44818110
AACGGGA20450.011.3307488
ACGGGAT16050.010.9335929
GAAACGG26800.010.6602276
AACGGGT5550.010.5388958
GGAAACG27050.09.89639955
CACCGCG5850.09.61546530
CCCCGAA3155.4686298E-69.3126391
ACCGCGC6251.8189894E-129.00019431
ACGCCCG3806.706079E-68.2892734
CCGCGCC6959.640644E-117.76995132
GCGTGAG18900.07.3811622
CGCGCCC4903.3031938E-67.347097433
ACAGGCG25600.07.293782718
GTTGGAA45800.07.2707562
CGTGAGC19550.07.250843523
CCCCGAC3500.00159400727.0919331
ACCCGGC6052.0478728E-77.06552336
Produced by FastQC (version 0.11.5)