FastQCFastQC Report
Sat 23 Apr 2022
EGAF00006070931

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006070931
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11033986
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT5650.025.48124744
CGTTTCG5750.025.03820833
TCTCGTA7150.020.45024143
TCGGAAT7150.020.13571437
TCGTATG7300.020.03974545
GTTTCGG7600.018.6473934
GATCGGA7550.017.9106311
TTCGGAA8100.017.77403636
ATCTCGT8350.017.51128442
ATCGGAA8400.016.6344782
GTCACGT8950.016.08592629
TTTCGGA9150.015.73439135
AGTCACG9250.015.5642228
ACACGTC10600.014.43085913
TCGGAAG9950.014.2685143
CGGAATC11150.013.71914738
CGTTGGA30950.013.6171921
CGTCTGA11200.013.05522916
AACGGGA28700.012.8543798
AAACGGG35250.012.8270297
Produced by FastQC (version 0.11.5)