FastQCFastQC Report
Sat 23 Apr 2022
EGAF00006070932

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006070932
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7553032
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGGGAT14150.012.876069
CGTTGGA21450.012.8235641
AACGGGA19000.012.5489298
AAACGGG22200.012.2598967
CGACGAT1300.002977921412.1403431
GAAACGG23200.012.0240676
CGGGATT13600.011.74285910
GGAAACG23950.011.5776665
CTCGTAT2851.5874284E-610.26013144
AGCGCAA1800.0029643499.9970512
CCACGAA4501.0235453E-89.0185391
ACGCTAG2000.007066158.99758435
CCCGACT3603.147623E-68.7680241
CCCGTTG2902.1481773E-48.5520641
CCCGGTT3401.3594666E-47.95753431
CGGCCTA2550.00567205447.939044539
CGCCCGG7405.456968E-127.90328335
CGGGAAT9550.07.772576310
CCCGAGT18350.07.74070841
CCCCGAA4101.8010942E-57.69875341
Produced by FastQC (version 0.11.5)