FastQCFastQC Report
Sat 23 Apr 2022
EGAF00006070933

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006070933
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13759504
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG6850.023.97601545
TCTCGTA8750.019.02822940
CTCGTAT8650.018.72798741
CGTATGC9150.017.70395543
ATCTCGT10450.015.5021739
TCGTATG11300.014.73371242
TCGGAAG12000.013.5068252
ATCGGAA12000.013.1447791
ACACGTC12900.013.081017512
CGTTGGA40300.012.1336431
AAACGGG39350.011.8358297
AACGGGA33650.011.501088
CGCGCCT4700.011.4888233
CGTCTGA14500.011.48171715
GAAACGG41950.011.0486296
ACGTCTG15800.010.96475314
AATCTCG16100.010.760715538
CGGGATT22500.010.50030610
ACCGCGC11150.010.49262631
ACGGGAT23700.010.3484049
Produced by FastQC (version 0.11.5)