FastQCFastQC Report
Sat 23 Apr 2022
EGAF00006070937

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006070937
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5545335
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT4700.023.44838144
GATCGGA4800.022.5623021
TCTCGTA4700.022.49130643
ATCGGAA5450.021.1013222
ATCTCGT5450.019.80885342
TCGTATG5350.019.80156545
TCGGAAG6750.017.0373633
ACACGTC6650.016.5725413
AATCTCG7600.014.7969141
CACGTCT7950.014.71129214
CGTCTGA7500.014.69431916
CGTTGGA16100.014.0138511
CGGAAGA8200.013.7496674
ACGTCTG8300.013.27799915
AAACGGG16900.011.3121947
GAAACGG16950.011.1489486
GCACACG10150.011.07945911
CGCGCCC3307.792551E-910.90684133
AACGGGA14050.010.565318
CACACGT10650.010.34811212
Produced by FastQC (version 0.11.5)