FastQCFastQC Report
Sat 23 Apr 2022
EGAF00006070939

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006070939
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8104878
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGCC600440.7408378016300801TruSeq Adapter, Index 1 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT67250.041.95361742
TCGTATG67800.041.6132943
TCTCGTA67950.041.52142741
CGTATGC68000.041.4906444
TCACGAT68750.040.97180635
ATCTCGT69800.040.4209340
ACGATCT71950.039.1810837
GATCGGA73500.039.0779081
GTATGCC72550.038.9815745
CACGATC72500.038.9151236
ATCGGAA73950.038.8247682
CATCACG72700.038.65308833
TCGGAAG75450.038.0824853
ACACGTC75400.037.98509613
CGTCTGA75700.037.77581416
ACGTCTG77150.037.1241615
CGGAAGA77700.037.0082134
GATCTCG76900.036.80599239
ATCACGA77300.036.41111434
CACGTCT78950.036.24859214
Produced by FastQC (version 0.11.5)