FastQCFastQC Report
Sat 23 Apr 2022
EGAF00006070943

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006070943
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13558494
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG5150.017.47424545
TCTCGTA6500.014.5390540
CTCGTAT6250.014.0394841
CGTATGC7200.012.81136543
CGTTGGA43700.012.6838661
AAACGGG44350.012.3268897
GAAACGG47100.011.8937636
AACGGGA38150.011.7947178
ACGGGAT25350.011.4489449
CGTCCGA1400.005264885311.2489216
GGAAACG49200.011.248925
TATCTCG7950.011.03863138
CGGGATT24450.010.76615610
ACGCCCG7300.010.48037934
CGCCCGG13600.010.42379235
TCGTATG8950.010.3063542
AACGGGT9050.010.1926568
CCGGCTA14050.09.76947538
ACCGCGC9050.09.69563131
CCCGTAA5651.8189894E-129.5710761
Produced by FastQC (version 0.11.5)