FastQCFastQC Report
Sat 23 Apr 2022
EGAF00006070948

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006070948
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9439946
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC5700.020.93691645
TCTCGTA5800.019.77987342
CTCGTAT5800.019.39234243
TCGTATG6400.017.57440244
CCGTATC6550.015.79809737
TATCTCG7150.015.73068440
TCCGTAT7550.015.49299736
CGTATCT7500.014.09693938
ATCTCGT8150.013.80053841
AGTTCCG7750.013.64212633
CGTTGGA26650.012.6095361
ACGGGAT17400.012.5394529
AACGGGA23350.012.2342548
GAAACGG30200.011.9919216
AAACGGG30300.011.8037427
TTCCGTA9550.011.77729735
GTACGCC1350.004017891411.66388834
GGAAACG30500.011.379895
CGGGATT16100.011.3165810
CGGGATA4304.8876245E-99.41586210
Produced by FastQC (version 0.11.5)