FastQCFastQC Report
Sat 23 Apr 2022
EGAF00006070949

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006070949
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6132043
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT5800.021.32492844
ATCTCGT5900.020.96366142
TCTCGTA5800.020.93720243
TCGTATG6700.018.51625345
AACGATC6700.018.46174238
ACGATCT6900.017.60024839
GATCGGA6800.017.2721391
GTCACGT7400.017.0212229
ATCGGAA7300.016.0756872
ACACGTC8200.015.63133913
ACGTGAA8600.015.16899532
AAACGAT8450.014.90445437
CACGTGA8350.014.81530531
TCGGAAG7950.014.7612023
CGTGAAA9250.014.10145633
AGTCACG8950.014.074128
CGTCTGA8850.013.72099816
GAAACGA9700.013.21563136
TGAAACG9750.013.14785935
CGTTGGA20650.012.9066531
Produced by FastQC (version 0.11.5)