FastQCFastQC Report
Sat 23 Apr 2022
EGAF00006070952

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006070952
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9657457
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTGGA28150.014.0324651
AACGGGA23600.013.7272438
ACGGGAT17700.013.6001399
AAACGGG29350.013.0312827
GAAACGG29650.012.975316
CGGGATT17600.012.39908510
GGAAACG32200.011.87789255
CGTAGAT2708.918934E-610.0320981
AACGGGT6100.09.9578778
CGTTAAC2057.5697794E-49.9097561
ACCGCGC6450.09.41747931
CGGGAAT10100.09.35531710
CACCGCG7750.09.28921130
CCCCGAT2801.4503075E-48.8676591
GACGATT6206.730261E-118.35602952
ACGGGAA10200.08.1608319
TGCGCCC6501.891749E-107.9607133
CCCCGTT4601.2189994E-67.85120771
GTTGGAA57550.07.78881842
ACAGGCG32250.07.743300418
Produced by FastQC (version 0.11.5)