FastQCFastQC Report
Sat 23 Apr 2022
EGAF00006070956

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006070956
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9046789
Sequences flagged as poor quality0
Sequence length36
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTGAG14850.013.02963122
CGTGAGC15250.012.49115923
GCCACCG12900.011.97645728
ACGGGAT15950.011.6607129
ACAGGCG20500.011.56037918
AACGGGA21300.011.4077368
CACCGCG5000.011.39972730
AAACGGG28650.011.3605457
CGTTGGA27950.011.1678311
CCCAAGT29350.010.7885151
GAAACGG29150.010.7544586
CCCTACG1408.1500315E-510.7191241
CCACCGC10700.010.65395129
ATAGGCG3101.8189894E-1210.64461218
CGGGATT15200.010.55854910
GGAAACG29600.010.5418025
GGCGTGA19000.010.49945821
CCCAAAG74950.010.47170451
GAGCCAC45800.010.41448226
AGGCGTG20200.010.17274320
Produced by FastQC (version 0.11.5)