FastQCFastQC Report
Sat 23 Apr 2022
EGAF00006070959

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006070959
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7604885
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGGGAT13100.011.5051239
AAACGGG21700.011.2993777
GAAACGG22900.011.2977766
AACGGGA18800.011.0082368
CGTTGGA22000.010.7570161
GGAAACG24450.010.2307915
CGGGATT12700.010.09622310
CTCGTAT3003.0568044E-69.74787344
TCGTATG3858.455045E-78.76835745
CGCCCGG6901.8189894E-128.47641235
CCGTATC3755.6273184E-68.39816838
CGGGAAT8050.08.38326410
TCTCGTA3909.524434E-68.07516143
AGTTCCG3909.524434E-68.07516134
GCCACCG17950.08.020534528
ACGCCCG3653.6419602E-58.011950534
TCCGTAT4354.8775055E-67.756928437
ACAGGCG25900.07.72997418
TATCTCG3501.9530806E-47.712603641
ACCGCGC5851.4026227E-87.690629531
Produced by FastQC (version 0.11.5)