FastQCFastQC Report
Sat 23 Apr 2022
EGAF00006070971

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006070971
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6362505
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGTCG300.005161490829.9851333
CTCGTAT7000.029.8785143
CGTATGC7550.028.37975345
TCTCGTA7400.028.2630142
TCGTATG7950.026.59100544
CGTCACG1350.024.98741127
ATCGGAA8800.024.9039331
GTCACGT8800.024.53078328
ATCTCGT8850.024.39353241
TCGGAAG8850.024.2436542
ACACGTC9450.023.31920212
AACGATC8800.023.25551237
AGTCACG7900.022.4886727
CGTCTGA9850.022.14394215
ACGATCT9950.020.79142238
CACGTCT10950.020.33014513
CGTGAAA10550.020.25062232
TCACGTG10900.020.01096729
TGAAACG10350.019.99008834
GAAACGA10700.019.96673635
Produced by FastQC (version 0.11.5)