FastQCFastQC Report
Sat 23 Apr 2022
EGAF00006070976

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006070976
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5096883
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTGGA16050.014.1946771
ACCGCGC3350.013.42835631
CGCGCCC2602.6830094E-912.97643933
AACGGGA15150.012.9164828
ACGGGAT10800.012.9123589
AAACGGG18750.012.8357177
GGAAACG18050.012.737185
GAAACGG18850.012.65127856
CGGGATT10200.012.12830610
CCGCGCC3950.011.95803532
CGCCCGG4800.011.24624735
ACAGGCG17750.010.51761818
TATGCCG1750.002306524710.3039153
CACCGCG4152.4374458E-1010.29776930
GCGTGAG13000.010.03737822
CCCCGTT2708.988636E-610.0252841
GCCACCG11250.09.9989228
ACGCCCG2302.0604809E-49.779345534
CGTGAGC13050.09.6541323
AGGCGTG16900.09.58260220
Produced by FastQC (version 0.11.5)