FastQCFastQC Report
Sat 23 Apr 2022
EGAF00006070978

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006070978
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5392082
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCCGA500.002270290622.492146
ACGTCCG1653.6379788E-1220.4479732
CGTCCGC1801.0913936E-1118.7439733
CGTTGGA16400.016.4129261
GTCCGCA2453.6379788E-1215.60519834
TCCGCAC2559.094947E-1214.99211735
AAACGGG18350.014.9494647
CGGGATT10100.014.916110
ACGGGAT12000.014.8029319
GAAACGG18550.014.7926746
AACGGGA15200.014.645148
GGAAACG19250.014.1899065
CGCACAT2702.1827873E-1114.15974737
GATCGGA2407.4578566E-1014.1371831
ACGCCCG2408.112693E-1014.05615334
AACGGGT3850.012.8488378
CTCGTAT2704.7275535E-912.49250444
ACGGGTT3304.9112714E-1112.2647999
CCCCGTA1400.005056813411.3097471
CCCAACG1400.005056813411.3097471
Produced by FastQC (version 0.11.5)