FastQCFastQC Report
Sat 23 Apr 2022
EGAF00006070980

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006070980
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6376976
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTGGA20400.014.5147621
AAACGGG20650.014.1556097
AACGGGA17850.013.7307278
ACGGGAT13700.013.13030059
CCCGGAT2256.971277E-813.0595931
GAAACGG21800.013.0019966
GGAAACG22000.012.8360165
CGGGATT13450.012.20410210
ACGGGTT3753.8198777E-1111.3927249
ACCGCGC4201.8189894E-1211.24308431
AACGGGT4651.8189894E-1210.6383688
CGGGAAT7850.010.31188610
CCTGCGA2201.2522124E-410.2741541
CGCGCCC3309.13642E-810.22082633
CACCGCG5101.10958354E-109.25908430
CCCGAAA2751.1744605E-49.0412561
ACCACGC12650.08.88781431
GTTGGAA38850.08.7810392
CCCATAA20400.08.6423781
CGGGTTT4702.2511813E-88.61152110
Produced by FastQC (version 0.11.5)