FastQCFastQC Report
Sat 23 Apr 2022
EGAF00006070982

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006070982
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4980294
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTGGA14250.012.4777931
CCTCGAT2351.299577E-712.4509091
TCGTATG3052.2555469E-911.8044443
AAACGGG14350.011.6026487
AACGGGA12200.011.24938
TCTCGTA2851.32688E-711.05294241
CTCGTAT3103.6148776E-810.88762142
GATCGGA2952.1265987E-710.6814841
CGCACCC2952.1369124E-710.67773133
GAAACGG15100.010.5793436
GGAAACG15600.010.5290365
ACGGGAT8650.010.4039779
CGGGATT9050.010.19273710
ACCCGGC5201.8189894E-129.95210436
CGATAAT3401.3930367E-79.929741
ACAGGCG17100.09.73623718
CCCGGTT2555.3915E-59.709081
CGTATGC3258.051702E-79.69345644
GCCACCG11000.09.61313328
GATTTCG6950.09.3886595
Produced by FastQC (version 0.11.5)