FastQCFastQC Report
Sat 23 Apr 2022
EGAF00006071003

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006071003
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9472524
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT5400.017.07816742
CGTTGGA30650.015.6805671
CGTATGC6050.015.61510944
TCTCGTA5950.015.12179441
AACGGGA26200.015.1103318
ACGGGAT19550.014.6123329
AAACGGG32400.014.2321797
GAAACGG33750.013.7961896
CGGGATT19600.013.54196310
GGAAACG35750.012.9864075
TCGTATG7150.012.89819543
ATCTCGT7450.011.77520840
GATCGGA8750.011.3463781
CGGGAAT12500.010.97679410
CATCTCG8700.010.3420839
ATCGGAA10150.010.2141232
CGTCTGA10000.010.12209716
GTTGGAA58950.09.6726632
AACGGGT6750.09.6639978
ACACGTC10300.09.39041213
Produced by FastQC (version 0.11.5)