FastQCFastQC Report
Sat 23 Apr 2022
EGAF00006071004

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006071004
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6816977
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACGGGA17450.014.308988
CGTTGGA22650.014.2719091
ACGGGAT13000.013.8429229
AAACGGG22250.013.5474987
GGAAACG25000.012.8733225
GAAACGG23400.012.7916686
CGGGATT11600.012.21697510
CGACAAT1903.7083728E-410.70787051
GATCGGA2657.0140977E-610.2364551
TCTCGTA3003.0557949E-69.74808241
CACCGCG6300.09.64329430
CCCGGAA3104.361549E-69.4797291
ACCGCGC6050.09.29794231
CGGGATA3155.690057E-69.28354610
CGGGAAT8450.09.05114110
CCCGATT2801.4271105E-48.88073351
GCGTGAG19000.08.64271922
GATTTCG9650.08.629175
CGAGGAA4202.9972398E-78.6116211
CGTGAGC18700.08.54078723
Produced by FastQC (version 0.11.5)