FastQCFastQC Report
Sat 23 Apr 2022
EGAF00006071005

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006071005
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7041367
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGGGAT12600.013.2121749
AACGGGA17200.013.2100978
CGTTGGA21200.012.8659671
CGGGATT12700.012.22245510
AAACGGG21950.012.09373957
GAAACGG23800.010.9679046
CCCCGAA2707.7042205E-710.8535661
GGAAACG25000.010.8079445
CGCGCCT2201.31365E-410.22563733
ACGCCCG4752.7284841E-119.94577934
GATTTCG8400.09.9180045
GCGTGAG16500.09.68027122
CGTGAGC16900.09.58426723
CGGGAAT8500.09.52788910
ACCACGC13250.09.50791531
ACAGGCG23750.09.4721718
CCCTAAA22450.09.2377111
CCACGAA4406.9867383E-99.2217371
CACCGCG4956.1299943E-109.08945630
CGTCAAT2300.0021912218.8207911
Produced by FastQC (version 0.11.5)