FastQCFastQC Report
Sat 23 Apr 2022
EGAF00006071009

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006071009
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6302356
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTGAG15500.015.09536422
CGTGAGC16250.014.81423
CGTTGGA18200.014.2620961
CGCGCCC2451.1004886E-913.77400433
AGGCGTG19850.013.14717920
ACCGCGC3600.013.12346131
ACAGGCG20350.012.93470418
CAGGCGT18950.012.70309619
CACCGCG4100.012.62047230
AAACGGG19150.012.4529467
GGCGTGA19700.012.44807621
AACGGGA14400.012.3423028
ACGGGAT11150.012.3079749
ACCACGC11050.012.21576231
GCCACCG12350.012.022881528
CCGCGCC3950.011.96071732
GAAACGG21050.011.3289276
CCCGTAA2201.0247681E-511.285661
CGCCCGG5800.011.24894935
GGAAACG21200.011.2487715
Produced by FastQC (version 0.11.5)