FastQCFastQC Report
Sat 23 Apr 2022
EGAF00006071015

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006071015
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6729276
Sequences flagged as poor quality0
Sequence length36
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA3450.019.160331
ATCGGAA3700.018.6720892
ACACGTC3850.017.91580613
CGTCTGA3800.017.7569416
TCGGAAG3950.016.7298623
CGGAAGA4900.014.4057334
ACGTCTG4850.014.22182515
CACGTCT5250.013.42387214
GCACACG5600.012.5848811
CACACGT6000.012.24571212
AACGGGA15650.011.8808258
ACGGGAT11950.011.7950339
CGTTGGA17150.011.6508311
AAACGGG18500.011.104227
CGGGATT11400.010.78569810
GAAACGG19300.010.5706476
CCTCGAT2002.6358066E-710.5164071
ATAGGCG1901.5884525E-610.25956618
GGAAACG20000.010.2127465
ACAGGCG17050.010.20170818
Produced by FastQC (version 0.11.5)