FastQCFastQC Report
Sat 23 Apr 2022
EGAF00006071021

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006071021
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10799064
Sequences flagged as poor quality0
Sequence length36
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA6250.022.3275971
GTCACCG7500.018.80126229
ACACGTC7850.017.77041213
ATCGGAA8200.017.1944452
CGTCTGA8650.016.6473616
TCGGAAG8900.016.0106053
CGGAAGA9300.015.16097454
ACGTCTG9600.014.84392315
TCACCGT10100.014.25858130
CACGTCT10900.013.07354614
CACCGCG7100.012.88836930
GCACACG11650.012.48895211
CGTTGGA24900.012.3536011
CACACGT12200.011.92592612
AACGGGA22650.011.3242678
ACGGGAT16050.011.1214189
AAACGGG27150.010.9942467
ACAGGCG27450.010.98358218
GCCACCG17650.010.70794328
GCGTGAG20350.010.61393222
Produced by FastQC (version 0.11.5)