FastQCFastQC Report
Sat 23 Apr 2022
EGAF00006071022

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006071022
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8149163
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTGGA29100.014.1143811
AACGGGA25450.013.0792848
ACGGGAT19100.013.0707259
GAAACGG30950.013.0083966
AAACGGG30550.012.8836687
CGGGATT17500.012.72351710
GGAAACG32150.012.2775195
GCGTGAG21850.011.42587922
CGTGAGC22100.011.39839723
ACCACGC14950.010.53203331
ACAGGCG28750.010.2482318
AACGGGT5800.010.0822028
CGCGCCC5007.2759576E-129.89637333
ACCGCGC6150.09.87515631
CAGGCGT27250.09.7393719
ACCGTGC7450.09.661631
GCCACCG17250.09.64940728
CACCGCG6300.09.64003430
ACGCCCA7650.09.40831634
CGCCCGG8450.09.31599735
Produced by FastQC (version 0.11.5)