FastQCFastQC Report
Sat 23 Apr 2022
EGAF00006071027

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006071027
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5995291
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTGGA19400.016.728521
AACGGGA16850.015.62088
ACGGGAT13300.015.5617039
AAACGGG20100.015.4454417
CGGGATT12200.015.12093310
GAAACGG21400.014.40805156
GGAAACG22550.013.8731135
CCCGGAT1953.785686E-511.5574531
ACCGCGC4800.011.24868731
CGGGATA2656.211376E-711.036262510
GCGTGAG17200.010.8561122
AACGCTA2301.7367915E-510.76104826
CGTGAGC18150.010.28788323
ACGCTAG2006.2117213E-410.12516927
AACGGGT4304.638423E-109.9403688
ACAGGCG24100.09.89461518
CACCGCG5500.09.81850930
GTTGGAA36950.09.5021772
CGGGAAT6900.09.45525910
AGGCGTG22000.09.20301120
Produced by FastQC (version 0.11.5)