FastQCFastQC Report
Sat 23 Apr 2022
EGAF00006071043

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006071043
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4860923
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGCC67830.13954139985348463TruSeq Adapter, Index 9 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC10100.035.40133344
GATCGGA10550.035.1225621
TCTCGTA10200.034.61653541
CTCGTAT10250.034.44767442
CGATCAG10250.034.44377533
ACGATCA10850.032.7469832
ATCGGAA11250.032.7214432
ATCTCGT10850.032.54273240
GTCACGA10700.032.3653929
TCGTATG11250.031.78547543
TCGGAAG11950.031.1833
TCACGAT11500.030.3094330
CGTCTGA12000.029.79459216
CGGAAGA12850.029.350484
ACACGTC12600.029.26812213
CACGATC12650.028.62063231
AGTCACG11950.028.60352528
ACGTCTG12750.028.394715
CACGTCT13850.026.78895214
GCACACG13100.025.74788311
Produced by FastQC (version 0.11.5)