FastQCFastQC Report
Sat 23 Apr 2022
EGAF00006071045

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006071045
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6792338
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGGGAT13350.013.9863919
AACGGGA18350.012.749888
CGTTGGA22650.012.5606651
AAACGGG22050.012.4468677
CGGGATT13550.012.11971510
ACCGCGC5450.011.970798531
GAAACGG22850.011.7157376
GGAAACG23700.010.8209495
CACCGCG6850.010.51079130
CCGCGCC6700.010.07395632
ACCCGGC5600.010.04508336
GCGCAAT2504.3631197E-59.8983220
CGCGCCT2057.793576E-49.87826333
CGTTGTA1850.003603329.7640171
CCGGCTA7450.09.66485438
ACGCCCG3502.1349479E-79.64306634
CGGCTAA7250.09.62040339
GCGTGAG15850.09.50991722
CGGGATA2658.258829E-59.33803810
GGCGCAA2952.6492045E-59.1509919
Produced by FastQC (version 0.11.5)