FastQCFastQC Report
Sat 23 Apr 2022
EGAF00006071049

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006071049
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5443127
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTGGA16450.012.1766331
ACCGCGC4001.0913936E-1111.2496131
GATTTCG6250.011.1600245
AAACGGG17450.010.7017337
CGCGCCC3002.6922135E-710.50011833
AACGGGA14050.010.4086058
GAAACGG18200.010.2607286
ACGCCCG3309.0474714E-810.22757534
CCGCGCC4355.711627E-109.82724632
GGAAACG18150.09.7934085
CTCGTAT2302.0509276E-49.78388744
AACGGGT3806.944174E-89.4730948
GCGCGAT2150.00120634999.41801720
CACCGCG4304.8657967E-99.41801730
CGGCTAA5751.8189894E-129.39166839
TCTCGTA2658.23429E-59.340543
CCGGCTA6550.09.27508438
GCCACCG12200.09.22073728
ACGGGAT10400.09.0859739
CCCGCAT2000.00703402059.00251
Produced by FastQC (version 0.11.5)