FastQCFastQC Report
Sat 23 Apr 2022
EGAF00006071056

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006071056
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7919992
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCCGA950.00538426214.2087156
ACGGGAT15800.013.9510119
AACGGGA21700.013.7856938
CGTTGGA25400.013.5001041
ACGCCCG3700.013.37388634
AAACGGG26800.012.4212027
GAAACGG27900.011.8533296
GGAAACG28450.011.7262085
CGGGATT14600.011.70839710
CGCCCGG7150.011.01028835
GCCCGGC14200.010.77109836
CGGGAAT9200.010.75725710
CGCCAAT1500.00861476510.5277131
CGGGATA3951.1295924E-910.249722510
ACCACGC14150.010.01430531
CACCACG15750.09.85383430
TGCCCGG8050.09.77932435
CCCTAAT17000.09.6872651
ACCGCGC4803.43789E-109.37185231
GCGTGAG20050.09.200706522