FastQCFastQC Report
Sat 23 Apr 2022
EGAF00006071057

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006071057
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2838533
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATGCCG48230.1699117114368584TruSeq Adapter, Index 3 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG7600.040.37259345
CTCGTAT8050.039.94625541
TCTCGTA8350.038.51106340
CGTATGC8700.036.70070643
CATCTCG8800.036.28620538
ATCTCGT8850.035.8271139
TCGTATG9350.034.1493242
ATCGGAA9600.033.9148671
TCGGAAG9800.033.2068442
ACACGTC10150.033.00673712
CGGAAGA10100.032.2205053
CGTCTGA10450.032.05917415
ACGTCTG10450.032.05917414
CACGTCT10850.031.084513
GTATGCC10500.030.83799644
GCACACG10950.030.59528510
CACACGT11700.028.63404811
CTTAGGC12400.026.8372332
TTAGGCA13750.023.38465733
AGAGCAC15000.023.2343467
Produced by FastQC (version 0.11.5)