FastQCFastQC Report
Sat 23 Apr 2022
EGAF00006071058

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006071058
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8776726
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT4200.015.53434444
TCTCGTA4100.015.36449743
TATCTCG4350.014.48123441
CGTTGGA25200.014.3305821
TCGTATG4900.012.85600945
GTCACGA4750.012.788053529
ACGGGAT15450.012.2315829
AACGGGA22800.011.8407248
CGGGATT15350.011.72501610
GGAAACG28050.011.4696425
AAACGGG28150.011.3489757
GATCGGA4900.011.0550191
GAAACGG28700.011.0530946
AGTCACG6050.010.78386528
CACGAGT5850.010.76803531
ATCTCGT6150.010.6088242
ACGAGTG6650.010.48767732
CACCGCG5650.09.95464730
ACCGCGC5900.09.5328431
CGGGAAT10900.09.49430510
Produced by FastQC (version 0.11.5)