FastQCFastQC Report
Sat 23 Apr 2022
EGAF00006071059

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006071059
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14662469
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTGGA45800.013.0786671
AACGGGA36700.012.1994898
ACGGGAT28250.012.1850599
CCGTACG3001.7444108E-911.999557533
AAACGGG46150.011.8958377
GAAACGG48050.011.753836
ACGCCCG6950.011.6537334
CGCGCCC8700.011.3788933
CGCCCGG13550.011.29059535
GGAAACG49500.011.2731565
CGGGATT26700.011.12276810
CGTACGT3451.3187673E-911.08605634
ACCGCGC11200.010.64703731
CACCGCG11950.010.16723130
CGTCCGA2057.793552E-49.8784256
CCGCGCC12150.09.44409632
GCCCGGC29300.09.06069836
GCCACCG31350.08.82763628
CGGGAAT16350.08.806684510
CCGGCTA13250.08.65967438
Produced by FastQC (version 0.11.5)